Impact of genomic variation on the gene regulatory networks of reprogramming to pluripotency

The reprogramming of somatic human cells to induced pluripotent stem cells (iPSCs) by only four transcription factors is one of the most striking remodelings of gene regulatory networks. Genetic variation is well known to modulate the regulatory network of pluripotency and contributes to the variability of cellular phenotypes and differentiation capacity of iPSC lines. In collaboration with drs. Chongyuan Luo and Kathrin Plath, and as part of the IGVF Consortium, we are developing methods to study the impact of diverse population genetics on the gene regulatory networks of reprogramming to pluripotency using population-scale single-cell joint profiling of RNA and DNA methylation and chromatin accessibility. Read more here.